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美国Worthington Biochemical Corporation成立于1947年,位于美国东海岸美丽海滨小城Lakewood, 有超过60年的生产经营历史,是世界上最主要的酶类供应商,是细胞分离酶的专家级公司。同时,也提供DNA/RNA纯化等分子生物学方面的产品。Worthington已经被购买和出售了好几次,并于1987年返回到沃辛顿家族。60年来,Worthington一直保持世界上高纯度酶的主要来源。
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  • question 胶原酶,不知道各类胶原酶有什么不同,选择的时候是以什么为依据?">【求助】请教不同胶原酶的类型

    • answer The first commercially available collagenase isolated from Clostridium histolyticum was offered by Worthington
      in 1959. At that time we offered one type of crude enzyme which we only tested for collagenase activity.
      Eventually, with the cooperation of many in the research community, four basic enzyme profiles were
      identified:
      Type 1 contains average amounts of assayed activities; collagenase, caseinase, clostripain and trypsin. It is generally recommended for adipocytes, epithelial, hepatocytes, lung and adrenal cell isolation.
      Type 2 contains higher protease contaminant levels, especially clostripain and trypsin. It is generally used for cardiomyocytes, bone, cartilage, muscle, thyroid and endothelial cell isolation.
      Type 3 contains lower levels of contaminating proteases. It is typically used for mammary and other soft tissues.
      Type 4 contains especially low trypsin activity. It is typically used for pancreatic islets and other applications where membrane protein and receptor integrity is crucial.
      CLSPA, Chromatographically purified collagenase, is specially purified to reduce the caseinase activity and provide high specific activity . It is typically used for pancreatic and parotid acini isolation and collagen structural analysis.
      Worthington also offers 0.22μm filtered collagenase preparations of each Type in 50 mg per vial pre-packaged form for direct reconstitution and use in cell isolation and culture procedures. Correlations between enzyme type and effectiveness with different tissues have been good, but not perfect, due in part to the variable parameters of use. Nevertheless most researchers consider the tissue-typing of crude collagenase lots to be a valuable service. A detailed description of the Worthington collagenase and contaminant assays can be found in the Worthington Enzyme Manual. In addition tissue
      specific references and detailed isolation conditions can be found in the Worthington Tissue Dissociation Guide.
      摘自www.worthington-biochem.com or www.tissuedissociation.com.
      你可以参考一下这个对你的实验有否帮助~
    • answer 不知楼上的朋友具体想做哪些实验,胶原酶确实有很多种,但好像也不是哪一种都经常用,作组织消化时还是主要就那么几种的,你可以把你要做的实验说出来,这样就可以很容易知道自己要用哪一种了。
    • answer 我是想知道选择的依据……很有用……谢谢
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